Microbial Phosphorylation of Pyridoxine

Microbial Transformation of Antibiotics: Phosphorylation

Addition of clindamycin to whole-cell cultures of Streptomyces coelicolor MUller resulted in the loss of in vitro activity against organisms sensitive to clindamycin. Incubation of such culture filtrates with alkaline phosphatase generated a biologically active material identified as clindamycin. Fermentation broths containing inactivated clindamycin yielded clindamycin 3-phosphate, the structu...

Microbial O-phosphorylation of macrolide antibiotics.

Crude enzymepreparations of Streptomyces coelicolor (Miiller), UC 5240 in the presence of ATP and Mg2+ catalyzed the conversion of oleandomycin, tylosin, leucomycin A3 and a mixture of spiramycins I, II and III to their antibiotically inactive I'-O-phosphates.10 Under identical conditions, erythromycin was converted to anhydroerythromycin-Z'-O-phosphate.10 As O-phosphorylation of aminocyclitols...

A case report of pyridoxine dependent seizure

pyridoxine dependent seizure have been recornized as a rare and important cause of intractable seizure in neonates and infants.prompt diagnosis and treatment can stop and prevent these seizure and consequential developmental disabilities.furthermore it can eliminate the unnecessary administration of anticonvulsant medications and thier side effects.the paitient was 7 months old infant who was r...

Use of borate to control the 5'-position-selective microbial glucosylation of pyridoxine.

Nearly 100% 5'-position selectivity of transglucosylation from maltodextrin to pyridoxine (PN) by cells of Verticillium dahliae TPU 4900 was observed when the reaction was carried out with borate. The same effect of borate was observed not only during synthesis of pyridoxine 5'-alpha-D-glucoside by partially purified enzyme of this strain but also during synthesis of this compound by other micr...

Pyridoxine responsive epilepsy: expanded pyridoxine dependency?